丹酚酸B在大鼠全血和血浆中的稳定性比较研究

Stability Comparison of Salvianolic Acid B in Whole Blood and Plasma

目的:采用HPLC-UV方法测定丹酚酸B在全血和血浆中的药物浓度变化情况,比较在两种介质中的丹酚酸B的浓度变化差异,考察丹酚酸B在这两种介质中的稳定性,为丹酚酸B的方法学优化提供依据,并规范含丹酚酸B血浆样品的处理方法。方法:静脉给予大鼠丹酚酸B后,于给药后不同时间取血测定血浆和全血中丹酚酸B的浓度;同时取空白全血和血浆,加入丹酚酸B标准品,放置不同时间后测定丹酚酸B的浓度。结果:大鼠静脉给予丹酚酸B后,药物浓度迅速下降,同一时间点取得的全血中所含药物浓度均显著低于血浆药物浓度(P<0.01)。制备含丹酚酸B的血浆和全血,放置相同时间的全血药物浓度显著低于同时间点的血浆药物浓度(P<0.01),全血药物浓度随放置时间增加显著降低(P<0.05),血浆药物浓度随放置时间增加无显著性降低。结论:丹酚酸B在血浆中的稳定性高于其在全血中的稳定性,建议在丹酚酸B的药代动力学研究中,取血后需要立即分离血浆,并尽快提取处理样品,避免人为误差导致血浆浓度偏低。

Object ive：To investigate the stability of salvianolic acid B（SAB） in plasma and whole blood.Methods：After SAB been injected intravenously into rats,blood samples were analyzed by an HPLC-UV method.SAB was added to the blank plasma or whole blood samples and bathed in water at 37℃.Then the samples were taken at different times to measure the concentration of SAB.Results：The concentrations of SAB were markedly reduced after injection.The differences of SAB concentration between the plasma and whole blood were significant at the same sampling times（P0.01）.In the in vitro study,the differences of SAB concentrations at different storage times were not significant in plasma,which were significant in whole blood（P0.05）.Conclusion：The stability of salvianolic acid B was higher in plasma than in whole blood.In the pharmacokinetic studies of salvianolic acid B,we should separate plasma from whole blood and process the samples as quickly as possible to avoid the occurrence of errors.