益肺通络颗粒的主要药效学研究

Pharmacodynamic study of Yifei Tongluo granule

目的研究益肺通络颗粒抗结核、抑制超敏、增强免疫及止咳等方面的作用。方法结核杆菌(H37RV)感染小鼠模型观察益肺通络颗粒的抗结核作用;2,4-二硝基氯苯(2,4-DNCB)对小鼠皮肤致敏和诱发,计算耳肿胀度;观察益肺通络颗粒的抗过敏作用,对小鼠炭粒廓清速率的影响,对小鼠氨水引咳试验的影响。结果中、高剂量益肺通络颗粒对结核杆菌感染小鼠肺、肝、脾脏器均存在明显的保护作用(P<0.01);对小鼠耳肿胀度有较好的抑制作用;小鼠炭粒廓清指数和校正廓清指数均显著高于空白对照组(P<0.01);能明显延长小鼠氨水引咳的潜伏期,减少3 min内咳嗽次数。结论益肺通络颗粒具有抗结核、抑制超敏、增强免疫及止咳的作用。

Objective To study the effects of Yifei Tongluo granule on anti-tuberculosis,inhibit hypersensitivity,immune function and antitussive action.Methods Mouse model infected by Mycobacterium tuberculosis H37RV was selected to observe the anti-tuberculosis effect of Yifei Tongluo granule.The anti-allergic effect of Yifei Tongluo granule was evaluated by observing the swelling of right ear of mouse smeared with 2,4-dinitrochlorobenzene.The effects of Yifei Tongluo granule on the carbon clearance index and the ammonia liquor induced cough mice were observed.Results The lung,liver and spleen protection functions of Yifei Tongluo granule were obviously increased in medium and high dose groups（P0.01）.Yifei Tongluo granule could markedly inhibited the ear swelling of mice.The carbon granule clearance index and correcting carbon clearance index in groups treated with Yifei Tongluo Granule were increased significantly than the blank control group（P0.01）.The cough incubation period of mice induced by ammonia liquor was significantly prolonged and the cough times in 3 minutes were decreased by Yifei Tongluo granule.Conclusion Yifei Tongluo granule has the function of anti-tuberculosis,inhibiting hypersensitivity,enhancing immune and relieving cough.