生物破乳菌Alcaligenes sp. S-XJ-1氮源利用特性

Characteristics of Nitrogen Source Utilization for Biodemulsifying Strain of Alcaligenes sp. S-XJ-1

以六种氨基酸及硝酸铵为氮源培养胞壁结合型生物破乳剂产生菌Alcaligenes sp.S-XJ-1,进而分析菌体对谷氨酸和硝酸铵的利用过程,并建立菌体的生长及氮源利用模型.结果表明:氮源种类和投量是影响菌体产量和性能的重要因素;菌体对氮源的利用顺序依次为有机氮、氨态氮、硝态氮;谷氨酸为氮源时对生物破乳菌产量的促进效果最佳,相比于硝酸铵为氮源时,菌体最大比增长速率和产率系数更高,用于菌体代谢供能的氮源消耗量更低.

Six animo acids and ammonium nitrate were tested to study the effects of nitrogen source on the growth of cell- bound biodemulsifying strain of Alcaligenes sp. S-XJ-1. And then the utilization process of nitrogen source was studied when glutamic and ammonium nitrate was separately used as nitrogen source. The growth model and nitrogen utilization model of S-XJ-1 were built up as well. The results show that S--XJ-1 prefers to use organic nitrogen when organic nitrogen and ammonium nitrogen coexist in the substrate, and ammonium nitrogen is preferred rather than nitrate nitrogen. Also, the yield and property of biodemulsifying bacteria were significantly affected by the variety of nitrogen source, and glutamic is testified to be the best nitrogen source for S-XJ-1 among the six amino acids. When glutamic is used as nitrogen source, S-XJ-1 exhibits a higher maximum specific microbial incremental rate and utilization ratio of nitrogen source, but a lower energy cost.