不同质量浓度脱落酸对小白菜硫代葡萄糖苷含量的影响

Effects of Different Concentrations of Abscisic Acid on Glucosinolates Contents in Pakchoi

为探讨外源激素对硫代葡萄糖苷(硫苷)的调控,研究了不同质量浓度(0、5、10和20 mg.L-1)脱落酸(ABA)处理对小白菜Brassica campestris L.ssp.chinensis(L.)Makino var.communis Tsen et Lee cv.Shanghaiqing生长和硫苷含量的影响.结果表明:不同质量浓度的ABA对小白菜鲜质量没有显著的影响.在10 mg.L-1的ABA处理时总脂肪族硫苷含量显著降低,然而5和10 mg.L-1的ABA处理显著诱导了总吲哚族硫苷和1-甲氧基吲哚-3-甲基硫苷含量的提高.20 mg.L-1的ABA处理显著增加了芳香族硫苷含量.但是10和20 mg.L-1的ABA处理显著抑制了小白菜总硫苷含量的增加.10 mg.L-1的ABA处理显著降低了总脂肪族硫苷在总硫苷中所占的比例,但显著增加了总吲哚族硫苷和芳香族硫苷的相对百分含量.研究表明,不同浓度ABA处理对小白菜单个硫苷和总硫苷含量及组成具有不同的影响.

To explore the regulatory effect of exogenous hormone on accumulation of the antitumor substance glucosinolate,plants of Shanghaiqing pakchoi（Brassica campestris L.ssp.chinensis（L.） Makino var.communis Tsen et Lee cv.Shanghaiqing） were treated by abscisic acid（ABA） with different concentrations（0,5,10 and 20 mg.L^-1）.The results showed that sprays of ABA solutions did not significantly affect biomass of pakchoi.10 mg.L^-1 ABA treatment significantly decreased the content of aliphatic glucosinolates in pakchoi shoots.However,treatments of 5 and 10 mg.L^-1 ABA significantly increased the contents of indole glucosinolates and neoglucobrassicin.The content of aromatic glucosinolate significantly increased by ABA at 20 mg.L^-1.But 10 and 20 mg.L^-1 ABA inhibited the increase in total glucosinolate content.In addition,10 mg.L^-1 ABA treatment decreased the portion of aliphatic glucosinolates in packoi shoots,increasing the relative percentages of indole glucosinolates and aromatic glucosinolate.It could be concluded that exogenous ABA with different concentrations have different effects on the contents andcomposition of glucosinolates.