摘要
用脲解链-离子交换柱层析-凝胶过滤等程序,自纯化过的小鼠珠蛋白中分离纯化β肽链。并做酶解肽段混合物的层析与电泳双向分离,获得肽斑图谱。依图谱上肽斑的分布位置,确定遗传型。通过肽斑性质的比较,鉴别小鼠代间遗传差异。取βTp3肽斑,用DABITC/PITC双偶合微量顺序测定法,测定氨基酸顺序,比较β肽链基因遗传密码变异位点。分析结果表明,3代SMMC/B小鼠具相同遗传型和群体遗传纯一性。
The separation and purification of beta chains of globm from the inbreeding SMMC/B mice were performed by the dissociation of chains in urea, CMhcellulose column chromatography for the separation of alpha and beta chains, and Sephadex G25 column chromatography for removing salts. The pure beta-chain was digested by trypsin. Digested fragments were resolved by cellulose membrane two-way electrophoresis, thus obtaining the map of spots of beta-chain fragments for B33, B34, Bp(), Bp and Bf. The spots,BTp3, which have the same positions ,were separated and micro-sequences were assayed by DABITC/PITC double coupling technique. According to the genetic code, we might deduce the nucleotide sequence of their mRNA from the known amino acid sequence of BTp3, a piece of beta-chain. By analyzing the altered genetic loci of beta-chain of globin, we might determine the genetic purity of the strain and distinguish objectively the genetic types between species and subspecies.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第3期244-247,共4页
Academic Journal of Second Military Medical University
关键词
珠蛋白
β肽链
肽斑图谱
遗传型
globin
betanchain
fingerprint
amino acid sequence
inbred strain of mice
genetic type