摘要
60只21日龄SPF混合雏鸡随机分为病毒感染组（I）和对照组（c）。I组雏鸡经眼、鼻途径感染传染性法氏囊病毒（IBDV），C组不做任何处理。两组雏鸡分别于病毒感染后的1、3、5、7、14、28d，每组随机抽取5只，心脏采血，分离血清，检测总超氧化物歧化酶（T—SOD）、谷胱甘肽过氧化酶（GSH—Px）、丙二醛（MDA）及一氧化氮（NO）含量。结果发现：21日龄sPF雏鸡感染IBDV后，其血清T—SOD和GSH—Px活性均于感染后5～7d显著或极显著低于对照雏鸡（P〈0．05或P〈0．01）；而MDA和NO含量分别于感染后5～7d和5d显著或极显著升高（P〈0．05或P〈0．01）。结果表明传染性法氏囊病病毒能够降低雏鸡血液抗氧化酶活性，促进自由基生成，从而影响雏鸡氧化一抗氧化能力。
Sixty 21-day-age SPF mixed chicks were randomly divided into infection group （I） and control group （C）. I group was infected with infectious bursal disease virus （IBDV） in eye and nose way, and C group didn＇t do any treatment. Respectively, 5 chicks which were extracted randomly from every group, then separated serum from heart and detected the contents of total superoxide dismutase （T-SOD）,gtutathione peroxidase （GSH-Px）,malonaldehyde （MDA）and NO after the two groups infected at 1,3,5,7,14,28 days. The results showed that when SPF chicks infected with IBDV ,T-SOD and GSH-Px activity in serum were all significantly or very significantly lower than the controls after infected 5 to 7 days （P〈0.05 or P〈0.01 ）;however, MDA and NO content were respectively raised significantly or very significantly after infected 5 to 7 days and 5 days （P〈0.05 or P〈0.01 ）. The results showed that IBDV could reduce chicks＇ antioxidant enzyme activity and promote the formation of free radicals so as to affect the oxidation-antioxidation ability of chicks.
出处
《中国家禽》
北大核心
2013年第11期16-19,共4页
China Poultry
基金
国家自然科学基金项目(30972162)
