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RNA结合蛋白LIN28A对肝癌细胞转移能力的影响 被引量：2

The effect of LIN28A on cell migration and invasion of hepatocellular carcinoma
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摘要 目的研究LIN28A基因在肝肿瘤细胞及二乙基亚硝胺(DEN)诱导的大鼠原发性肝癌中的表达变化,揭示其在肝癌发生发展过程中的作用。方法利用实时荧光定量PCR(RT-PCR)检测肝癌细胞及DEN诱导的大鼠原发性肝癌模型中LIN28A信使核糖核酸(mRNA)的表达;以携带LIN28A的慢病毒(lentivirus)载体感染肝癌细胞,侵袭小室实验(transwell assay)检测过表达LIN28A对肿瘤细胞转移能力的影响。结果 RT-PCR结果显示,LIN28A在肝癌细胞及大鼠原发性肝癌中表达量上调;侵袭小室实验表明,过表达LIN28A能明显提高肝癌细胞的迁移和侵袭能力。结论 LIN28A在肝癌细胞中的表达升高,对肝癌细胞的转移能力具有促进作用。 Objective To analyze the mRNA levels of LIN28A in liver cancer cell lines and DEN-induced rat hepatocellular carcinoma(HCC) samples,and study the function of LIN28A in tumorigenesis of HCC.Methods Examine the mRNA expression of LIN28A gene in liver cancer cells and DEN-induced rat HCC samples by real-time PCR.Detect the effect of LIN28A on cell migration and invasion of HCC.Results Real-time PCR showed that the expression of LIN28A was upregulated in twelve liver cancer cell lines and DEN-induced rat HCC.Transwell assay revealed that LIN28A overexpression significantly enhanced the cell migration and invasion of HCC.Conclusion Upregulation of LIN28A may be important for metastasis of HCC.
作者 易敏 许文萍 李倩倩 丁晨虹 陈斐 张新
机构地区 华东理工大学生物工学院 第二军医大学上海长征医院消化内科
出处 《国际消化病杂志》 CAS 2013年第3期189-192,共4页 International Journal of Digestive Diseases
基金 国家自然科学基金面上项目(81071842) 上海市科委"创新行动计划"基础研究重点项目(10JC1411900)
关键词 LIN28A 肝癌 细胞迁移 细胞侵袭 LIN28A Hepatocellular carcinoma Cell migration Cell invasion
分类号 R735.7 [医药卫生—肿瘤]
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参考文献14

  • 1Lehrbach N J,Armisen J,Lightfoot HL,et al.LIN-28 and the poly(U) polymerase PUP-2 regulate let-7 microRNA processing in Caenorhabditis elegans.Nat Struct Mol Biol,2009,16:1016-1020.
  • 2Balzer E,Heine C,Jiang Q,et al.LIN28 alters cell fate succession and acts independently of the let-7 microRNA during neurogliogenesis in vitro.Development,2010,137:891-900.
  • 3Sakurai M,MiKi Y,Masuda M,et al.LIN28:a regulator of tumor-suppressing activity of let-7 microRNA in human breast cancer.J Steroid Biochem Mol Biol,2012,131:101-106.
  • 4Viswanathan SR,Powers JT,Einhorn W,et al.Lin28 promotes transformation and is associated with advanced human malignancies.Nat Genet,2009,41:843-848.
  • 5Guo Y,Chen Y,Ito H,et al.Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma.Gene,2006,384:51-61.
  • 6Burrows C,Abd Latip N,Lam SJ,et al.The RNA binding protein Larp1 regulates cell division,apoptosis and cell migration.Nucleic Acids Res,2010,38:5542-5553.
  • 7Polesskaya A,Cuvellier S,Naquibneva I,et al.Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiency.Genes Dev,2007,21:1125-1138.
  • 8Wang Q,Hu0ng J,Zhang X,et al.The spatial association of gene expression evolves from synchrony to asynchrony and stochasticity with age.PLoS One,2011:6:e24076.
  • 9Nam Y,Chen C,Gregory RI,et al.Molecular basis for interaction of let-7 microRNAs with Lin28.Cell,2011,147:1080-1091.
  • 10Peng S,Maihle NJ,Huang Y.Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer.Oncogene,2010,29:2153-2159.

同被引文献24

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国际消化病杂志
2013年 第3期

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