慢传输型便秘大鼠结肠诱导型一氧化氮合酶和血红素氧合酶2的变化

Alteration of inducible nitric oxide synthase and heme oxygenase-2 in the colon of slow transit constipation rats

目的探讨慢传输型便秘(STC)大鼠结肠内诱导型一氧化氮合酶(iNOS)和血红素氧合酶2(HO-2)的变化。方法健康Wistar大鼠32只,随机分为STC组和对照组,每组16只。采用复方苯乙哌啶灌胃法制备STC大鼠模型,饲养100 d后,采用活性炭灌胃法测定肠道传输速度确定模型建立。用免疫组织化学方法分别检测iNOS和HO-2在大鼠结肠的表达情况。结果 STC组大鼠日均粪便粒数、日均粪便干重、日均粪便质量均比对照组明显减少;检测大鼠肠道传输速度,STC组较对照组明显减慢,首粒黑便排出时间较对照组显著延长。iNOS和HO-2在STC大鼠结肠的表达明显强于对照组。结论 iNOS和HO-2在STC大鼠结肠的表达异常,表明iNOS和HO-2在慢传输型便秘发病机制中可能起着重要的作用。

Objective To investigate alteration of inducible nitric oxide synthase （iNOS） and heine oxygenase2 （HO-2） in the colon of slow transit constipation （STC） rats. Methods Thirty-two healthy Wistar rats were randomly divided into control group and model group of STC. The rats in model group received daily diphenoxylate by intragastric administration to develop the STC model. 100 days later, intestinal transit functions were examined by activated charcoal pushing test. Meanwhile, the expressions of iNOS and HO-2 were detected by immunohistochemistry. Results The fecal data of model rats was significantly smaller than that of the normal rats. The movement of contents from the proximal to the distal colon and rectum in model group were significantly slower than those in normal group. In model rats, the time of discharge of the first black fecal was significantly longer than that in normal rats. The expressions of iNOS and HO-2 in model group were significantly higher than that in control group. Conclusion The abnormal expressions of iNOS and HO 2 in the colon of STC model rats suggest that iNOS and HO-2 may play important roles in the pathogenesis of STC.