罗格列酮对哮喘大鼠引哮喘发作潜伏期的影响

Effect of rosiglitazone on the latent period of asthma in rats with asthma

目的观察过氧化物酶体增殖物活化受体γ(PPARγ)配体罗格列酮(RSG)对支气管哮喘(哮喘)大鼠气道哮喘发作潜伏期的影响。方法 SD大鼠75只,分为正常对照组(A组)、哮喘组(B组)、RSG治疗组(C组),每组25只。制备各组大鼠体外去上皮气管环,比较在0.05mmol·L-1、0.5mmol·L-1、5.0mmol·L-1浓度梯度乙酰胆碱(ACh)激发下,各组大鼠体外气管环收缩张力大小。观察三种浓度0.01mmol·L-1、0.1mmol·L-1、1.0mmol·L-1RSG对A、B两组大鼠体外气管环的ACh量效曲线的影响,比较各组大鼠引喘潜伏期差异。结果三组大鼠引喘潜伏期分别为A组(121.50±17.44)s,B组(61.50±17.68)s,C组(94.40±21.17)s,差异有统计学意义(P<0.05,n=20)。三组大鼠气管环对三种浓度ACh的反应率,B>C>A组,差异有统计学意义(P<0.05,n=20)。RSG可引起A、B两组大鼠体外气管环ACh量效曲线右移。结论 RSG可延长哮喘发作潜伏期,降低气道收缩张力,可能具有减轻气道高反应性、减少哮喘发作的作用。

Objective To observe the function of peroxisome proliferator activated receptor（PPARγ） and rosiglitazone （RSG） on the experimental rats bronchial asthma （known as asthma ） airway hyperresp-onsiveness. Methods 75 male SD rats were randomly divided into 3 groups, control group （ group A）, the asthma group（ group B）, RSG treatment group（ group C） , each group contains 25 experimental rats. The latent period of asthma in all groups were measured. Isolated tracheal rings of rats, without epithelium, were prepared. The contraction tension strength which were induced by the concentration gradient of ACh （0.05 mmol·L^-1 , 0.5 mmol·L^-1 , 5.0 mmol·L^-1 ） on the isolated tracheal rings in three groups were compared. The effect on the ACh dose-response curves of isolated tracheal rings in rats from group A and group B caused by three different concentration gradient of RSG, 0.01 mmol·L^-1 , 0.1 mmol·L^-1 and 1.0 mmol·L^-1 were observed. Results 3 groups＇ incubation period of asthma were 121.50 ± 17.44 s （ group A）, 61.50 ± 17.68 s （group B） , 94.40 ±21.17 s （group C） , and there were significant differences （P 〈0.05, n =20）. The response rate of tracheal rings in rats from three groups , B 〉 C 〉 A, and there were significant differences （P 〈 0. 05, n = 20）. With the RSG, ACh dose-response curves of isolated tracheal shift significantly to the right side in rats from group A and group B. Conclusions RSG can extend the incubation period of asthma, lower the airway contraction. Reduce the airway hyperresponsiveness and asthma attack.