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HMGCS1基因对猪肌肉生长发育的影响及miRNA-18a/b对HMGCS1基因的调控 被引量：6

Effects of HMGCS1 Gene on Skeletal Muscle Growth and Development and Regulated by miR-18a/b of Pig
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摘要 【目的】研究HMGCS1基因对猪骨骼肌生长发育的影响及其靶标的验证。【方法】采用real-time PCR对通城猪不同组织以及背最长肌不同发育时间点的HMGCS1基因的表达变化进行检测。【结果】①HMGCS1基因在成年猪肺组织中的表达量最高;②在背最长肌发育中,该基因分别在胚胎期33和65 d高表达,出生后20、30、40和60 d低表达;③双荧光素酶试验显示,转染miR-18a/b minics组荧光素酶活性低于对照组。【结论】HMGCS1参与了猪骨骼肌生长发育过程,并受miR-18a/b的调控,是猪的产肉性状的潜在候选基因。 【Objective】The purpose of the present research was to explore whether HMGCS1 impacts the skeletal muscle growth and development of pig and validate its target gene.【Method】In this study,the expression of HMGCS1 in different tissues and the expression change during Longissimus dorsi muscle development（28 stages） was investigated by real-time qPCR.【Result】 The result showed that the HMGCS1 gene was strongly expressed in lung of adult pig,and the expression level of HMGCS1 gene in embryonic period 33 d and 65 d were higher than those in other periods in Tongcheng pigs,and lower expression at postnatal 20 d,30 d,40 d and 60 d.The luciferase activity was lower in miR-18a/b minics than in the control.【Conclusion】The results of study indicated that HMGCS1 regulates skeletal muscle growth and development by miR-18a/b,and is a potential candidate target gene of meat production traits.
作者 孙伟 唐中林 谭林 雷初朝 李奎
机构地区 西北农林科技大学动物科技学院 中国农业科学院北京畜牧兽医研究所/农业部畜禽遗传资源与利用重点开放实验室
出处 《中国农业科学》 CAS CSCD 北大核心 2013年第12期2543-2549,共7页 Scientia Agricultura Sinica
基金 国家自然科学基金项目(31171192 31172182) 国家重大科学研究计划(2012CB124706-6)
关键词 猪 HMGCS1基因 miR-18a b 骨骼肌 生长发育 pig HMGCS1 gene miR-18a/b skeletal muscle growth and development
分类号 S828 [农业科学—畜牧学]
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参考文献23

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共引文献102

同被引文献78

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中国农业科学
2013年 第12期

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