鸡催乳素受体基因多态性与就巢及产蛋性状的关联分析

Polymorphism of Chicken Prolactin Receptor Gene and It’s Association with Broodiness and Egg Reproduction Traits

【目的】揭示与繁殖性状关系密切的催乳素受体(prolactin receptor,PRLR)基因与中国地方鸡种的就巢性及产蛋性能的关系。【方法】本试验采用PCR-SSCP方法,以576只宁都黄鸡为材料,检测PRLR基因的多态性,并进行多态性与就巢和产蛋性状间的关联分析。【结果】证实了前人研究过的SNP1(T10862C,外显子3)和SNP2(T25670C,外显子6)均与就巢性状无显著关联(P>0.05);SNP2对300 d产蛋量影响显著(P<0.05);新发现的SNP3(G30716A,内含子8)显著影响开产日龄(P<0.05);新发现的SNP4(A31900G,外显子10)极显著地影响就巢率(P<0.01),显著影响就巢天数和300 d产蛋量(P<0.05)。由4个SNPs构建的不同单倍型在开产日龄上的差异达到极显著水平(P<0.01),在其它性状上的差异不显著(P>0.05)。【结论】PRLR基因的多态性与就巢和产蛋性状相关,SNP4的效应更为突出。

【Objective】 In order to reveal the relationship between bloodiness and egg reproduction of Chinese indigenous chicken and prolactin receptor（PRLR） gene,which closely related to reproductive traits among amimals.【Method】Using the PCR-SSCP method and 576 Ningdu Yellow chicken,the experiment studied the polymorphism of PRLR gene and its association with nesting and egg production traits.【Result】The results confirmed previous studies that SNP1（T10862C,exon 3） and SNP2（T25670C,exon 6） had no significant association with broody traits（P0.05）.The effect of SNP2 on egg production of 300 d（EN-300 d） was significantly（P0.05）.The new SNP3（G30716A,intron 8） significantly influenced the age at first egg（AFG）（P0.05）.The novel SNP4（A31900G,exon 10） had an extremely remarkable（P0.01） impact on the nesting rate,significant（P0.05） impact on the total broody days and EN-300 d.The differences among different haplotypes constructed by the four SNPs,in AFG reached an extremely significant level（P0.01）,in other traits were no significant differences（P0.05）.【Conclusion】 PRLR gene polymorphism was associated with broodiness and egg production traits,especially the effect of SNP4.