棉花黄萎病病原菌诱导侵染对转基因抗病棉花生理性状的影响

The Effect of Physiological Traits of Transgenic Cotton Induced by Verticillium wilt Pathogen

通过研究棉花黄萎病病原菌诱导侵染对转基因抗病棉花生理性状的影响,旨在为提高转基因抗病棉花抗病性提供理论依据。Byd、28p两品种棉花为供试材料,应用花粉管通道法将几丁质酶与β-1,3-葡聚糖双价抗病基因导入棉花株系中,多年筛选获得不同抗性水平的棉花株系;棉种经棉花黄萎病病菌侵染后,苗期测定棉株内的生理生化变化。结果显示,转基因抗病棉株体内的酶活性已达对照水平,而感病及耐病棉株内的酶活性均有不同程度的变化。所测定的棉株内的酶活性及脯氨酸含量的变化与棉株的抗病性相关。

To provide theoretical basis for improving resistant in transgenic cotton by researching the effect of physiological traits of transgenic cotton induced by Verticillium wilt pathogen.Chitinase and β-1,3-glucan of bivalent resistance genes were delivered to two cotton lines of Byd,28p by pollen-tube pathway method.The transgenic cotton lines with different resistant levels were obtained by many years screening.After the Verticillium wilt pathogen infestation to cotton seeds,the physiological and biochemical changes in transgenic cotton were determined at seedling stage.The results showed that the enzyme activity in transgenic disease-resistant cotton plants had reached the control＇s level,however,the level of enzyme activity in susceptible and disease-resistan cotton plants were different.The enzyme activity and changes of proline content were related to disease resistance of transgenic cotton plants.