Box-Behnken响应面设计法优化黑木耳菌质多糖提取工艺

Optimum Technique of Extracting Mycelium Polysaccharide from Auricularia auricular Using Box-Behnken Design-Response Surface Methodology

为了优化黑木耳菌质多糖的提取工艺,采用Box-Behnken设计方法,研究水提温度、水提时间、液料比及其交互作用对黑木耳菌质多糖得率的影响。应用Design Expert软件和响应面分析相结合的方法,模拟得到回归方程的预测模型和可信度,分析得到最佳的提取条件为:水提温度81.59℃,水提时间81.40 min,液料比36.42∶1。黑木耳菌质多糖得率的预测值为7.92 mg/g,采用上述最佳提取条件进行验证试验,测得菌质多糖得率为7.79 mg/g。采用响应法优化得到的黑木耳菌质多糖提取条件参数准确可靠,该研究为黑木耳菌质多糖的工业化生产提供了必要的技术支持。

To optimize the extraction technique for mycelium polysaccharide from A.auricular,the effects of extraction temperature,extraction time,the liquid to solid ratio and their interaction on extraction rate were studied by Box-Behnken design.The predictive model and reliability were developed by Design Expert software and response surface analysis（RSM）.The optimal extraction conditions were achieved and listed as follows ：extraction temperature 81.59℃,extraction time 81.40 min,liquid-to-solid ratio 36.42∶1.Under the optimum conditions,the extraction yield of mycelium polysaccharide from A.auricularreached 7.79 mg/g,while the predicted one was 7.92 mg/g.The results showed the suitability of RSM in optimizing the extraction of mycelium polysaccharide from A.auricular.The necessary technical support was provided by this experimental study for the industrial production of mycelium polysaccharide fromA.auricular.