葛花粉体高效球磨制备技术及其醒酒活性测定

High efficient ball milling technology and determination alcohol metabolism of Flos puerariae

为探究葛花粉体粒径与功效释放速度的相关性及其醒酒机理,通过比较4种不同粉碎方法处理得到的植物本体、常规粉体、Ⅰ级粉体、Ⅱ级粉体在总物质溶出度、黄酮溶出量及乙醇脱氢酶(ADH)体外活性实验方面的差异性,发现4种粉体均可在体外激活乙醇脱氢酶,但在总溶出度上Ⅱ级粉体优于Ⅰ级粉体,其次是常规粉体和植物本体,其瞬时溶出度可达到植物本体的8.6倍。在黄酮溶出量和ADH激活率上也呈现相同趋势,Ⅱ级粉体黄酮溶出含量较植物本体提高57.98%,激活率为本体的2倍。实验表明,采用高效球磨技术制备的葛花Ⅱ级粉体较其他粉体均呈现出最优实验结果,且其醒酒作用机制与其能激活乙醇脱氢酶有关,为开发新型醒酒功能食品提供理论依据。

The mechanism for Flos puerariae facilitating alcohol metabolism was studied.The relativity between particle size and the release rate of efficacy was demonstrated.The activity of alcohol dehydrogenase（ADH） in vitro and the dissolution of total material and flavonoids from four kinds of powder of Flos puerariae which used different methods to shatter were compared.The results showed that four kinds of powder could activate ADH,but grade Ⅱpowder had an obvious advantage over grade Ⅰpowder in the total dissolution test.Its instant dissolution can reached 8.6 times of that of plant body.There was the same trend in the amount of flavonoids dissolution and the activation rate of ADH.The amount of flavonoids dissolution in grade II powder was 57.98% higher than that in plant body and activation rate was 2 times of that in it.The grade Ⅱ powder which was gained by high efficient ball milling technology had a more beneficial effect on these tests than other powder.It was also indicated that it was useful to select Flos puerariae to facilitate alcohol metabolism,this new efficient technology provided a theoretical basis for the development of new sober up functional foods.