脆弱类杆菌NCTC9343脂多糖的生物学活性

Biological Characterization of the Lipopolysaccharide (Endotoxin) Extracted from Bacteroides fragilis NCTC 9343

本文报道经动物实验检测脆弱类杆菌9343株脂多糖(LPS)的生物学活性,发现该LPS具有较典型的内毒素活性,如对家免的致热性;对鸡胚、小鼠的致死性;引起局部Shwartzman反应的能力和对鲎试剂的致凝性。此LPS的生物学活性低于革兰氏阴性肠杆菌LPS的生物学活性,其原因与此LPS类脂A分子上不完全脂肪酸酰化有关。

In this study, biological activities of the lipopolysaccharide (LPS) extracted from Bacteroides fraglis NCTC 9343 were measured in animals. The results showed that LPS expressed typical endotoxicities, e.g. pyrogenicity in rabbits, lethality in mice and chick embryoes, ability to induce local Shwartzman reaction and coagulability for limulus emoebcyte lysate. But its biological activities were lower compared with that of Enterobacteriaceae. It is suggested that the weaker biological activities are related to the unusual chemical structure of the LPS.