桦褐孔菌多糖对急性酒精性肝损伤模型大鼠的保护作用研究

Study on Protective Effect of Inonotus obliquus Polysaccharide on Acute Alcoholic Hepatic Injury Model Rats

目的:研究桦褐孔菌多糖对急性酒精性肝损伤模型大鼠的保护作用。方法:连续灌胃白酒(20ml/kg)9d以复制大鼠急性酒精性肝损伤模型。健康雄性Wistar大鼠48只随机均分为空白对照(等容生理盐水)、模型(等容生理盐水)、海王金樽(270mg/kg)与桦褐孔菌多糖高、中、低剂量(600、400、200mg/kg)组,灌胃白酒1h后灌胃给药,每天1次,连续9d。末次给药后,取大鼠血液,检测血清天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT);取肝组织,HE染色后进行病理形态学检查。结果:与空白对照组比较,模型组大鼠血清AST、ALT活性显著增强(P<0.01);与模型组比较,桦褐孔菌多糖高、中、低剂量组大鼠血清AST、ALT活性显著减弱(P<0.01)。与模型组比较,桦褐孔菌多糖高、中、低剂量组大鼠肝脏变性、炎症坏死均有所减轻。结论:桦褐孔菌多糖具有良好的解酒、护肝作用。

OBJECTIVE： To observe the protective effects of Inonotus obliquus polysaccharide on alcoholic hepatic injury in rats. METHODS：Acute alcoholic model of rats were induce by i.g. administration of white spirit. 48 health male Wistar rats were randomized into blank control group （constant volume of normal saline）, model group （constant volume of normal saline）, Haiwang jinzun group （270 mg/kg） and I. obliquus polysaccharide high-dose, medium-dose and low-dose groups （600, 400, 200 mg/kg）. 1 h after, they were given relevant medicine intragastrically once a day for consecutive 9 days. After last administration, the blood samples of rats were collected to measure AST and ALT, and then hepatic tissue of rats were collected to conduct HE staining and pathological examination. RESULTS： Compared with blank control group, the activities of AST and ALT increased significantly in model group （P〈0.01）; compared with model group, the activities of AST and ALT decreased significantly in I. obliquus polysaccharide high-dose, medium-dose and low-dose groups（P〈0.01）. Hepatic degeneration and inflammatory necrosis both were relieved in I. obliquus polysaccharide high-dose, medium-dose and low-dose groups. CONCLUSIONS：I. obliquus polysaccharide shows good anti-inebriation and liver-protective effects.