正交试验优选黄蜀葵花中总黄酮的提取工艺

Optimization of the Extraction Process for Total Flavonoids from Abelmoschus manihot by Orthogonal Test

目的:优选黄蜀葵花中总黄酮的提取工艺。方法:采用正交试验法,以槲皮素含量为评价指标,以回流时间、回流温度、酸化液量、酸化液配比(甲醇∶25%盐酸)为考察因素,优选酸化工艺;以乙醇体积分数、乙醇用量、提取时间、提取次数为考察因素,优选总黄酮提取工艺。结果:最佳酸化工艺为用25倍量甲醇-25%盐酸(4∶1,V/V)酸化液,70℃水浴回流0.5h;最佳提取工艺为用12倍量85%乙醇,回流提取2次,每次2.5h。结论:优选的工艺合理、可行,可用于黄蜀葵花中总黄酮的提取。

OBJECTIVE： To optimize the extraction process of total flavonoids of Abelmoschus manihot. METHODS： The acidification technology was optimized by orthogonal experiment with the content of quercetin as index using reflux time, reflux temperature, amount of acid fluid, ratio of acid fluid （ethanol ∶ 25% hydrochloric acid） as factors. The extraction technology was optimized using ethanol concentration, ethanol consumption, extraction time and extraction times as factors. RESULTS： Optimized acidification technology was as follows： 25-folds of ethanol-25% hydrochloric acid （4 ∶ 1,V/V） as acid fluid, 70 ℃ water bath for refluxing 0.5 h; optimized extraction technology was as follows： 12-folds of 85% ethanol, extracting 2 times,2.5 h each time. CONCLUSIONS：Optimized technology is reasonable and feasible, and can be used for the extraction of total flavonoids from A. manihot.