摘要
目的:制订红背桂药材的质量标准,为其开发利用提供科学依据。方法:分别对红背桂药材的性状、显微特征进行观察鉴别;按照《中国药典》方法对10个不同产地药材中浸出物、灰分和水分进行测定;以没食子酸为对照品,采用薄层色谱(TLC)法对其进行定性鉴别;采用高效液相色谱法测定药材中没食子酸的含量:色谱柱为迪马C18(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸水(10∶90,V/V),检测波长为273nm。结果:对红背桂药材的性状、显微特征进行了描述。暂定红背桂药材中总灰分不得过11%,不溶性灰分不得过3%,水浸出物不得少于12%,醇浸出物不得少于14%,水分不得过12%。TLC中主斑点清晰、分离度好。没食子酸的进样量在0.0095~1.9000μg范围内与其峰面积积分值呈良好的线性关系(r=0.9999);精密度、稳定性、重复性试验的RSD均<2%;平均加样回收率为101.93%,RSD=1.67%(n=6)。结论:所建标准可用于红背桂药材的质量控制。
OBJECTIVE: To provide scientific basis for the utilization and development of Excoecaria cochinchinensis by setting up the quality standard of E. cochinchinensis. METHODS: The property and microscopic characteristics of E. cochinchinensis were determined respectively. Extract, moisture and ash of medicinal material from 10 producing areas were determined according to Chinese Pharmacopeia; E. cochinchinensis was qualitatively determined by TLC using gallic acid as control; the content of gallic acid was determined by HPLC: the determination was performed on Diamonsil C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-0.1% phosphoric acid (10 ∶ 90,V/V). The detection wavelength was set at 273 nm. RESULTS: The property and microscopic characteristics of E. cochinchinensis were described. The contents of total ash, acid-insoluble ash, aqueous extract, ethanol extract and moisture were no more than 11%, 3%, 12%, 14% and 12%. TLC spots were clear and well-separated without interference from negative control. The linear range of gallic acid were 0.009 5-1.900 0 μg(r=0.999 9); RSDs of precision, stability and reproducibility tests were all lower than 2%; average recovery rates were 101.93%(RSD=1.67%,n=6). CONCLUSIONS: The established standard can be used for the quality control of E. cochinchinensis.
出处
《中国药房》
CAS
CSCD
2013年第27期2542-2545,共4页
China Pharmacy
基金
广西科学研究与技术开发计划项目(No.10100024-4)