黄柏-苍术药对不同比例配伍中生物碱含量的变化

Content Change of Alkaloids in Different Compatibility Proportions of Phellodendron chinense and Atractylodes lancea

目的:探讨中药药对黄柏-苍术不同比例配伍中生物碱含量的变化规律。方法:采用高效液相色谱法测定黄柏-苍术药对不同比例配伍中黄柏碱、小檗碱的含量。色谱柱为WatersC18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸水溶液(梯度洗脱),检测波长为285nm。结果:黄柏碱、小檗碱的质量浓度分别在0.0206～0.4112、0.0211～0.4220mg/ml范围内与各自峰面积积分值呈良好的线性关系(r均为0.9997);二者精密度、稳定性、重复性试验的RSD均<1%;平均加样回收率分别为99.39%、99.51%,RSD分别为1.62%、1.05%(n均为6)。结论:黄柏与苍术配伍时,其生物碱含量变化规律为随苍术的增加而减少。

OBJECTIVE： To explore the variation of alkaloids content in the compatibility of different proportions of Phellodendron chinense and Atractylodes lancea. METHODS： HPLC method was used to determine contents of phellodendrine and berberine in different proportions of P. chinense and A. lancea. The determination was performed on Waters C18（250 mm×4.6 mm,5 μm） with mobile phase consisted of acetonitrile-0.1% phosphoric acid （gradient elution）. The detection wavelength was at 285 nm. RESULTS：The linear range of phellodendrine and berberine were 0.020 6-0.411 2 mg and 0.021 1-0.422 0 mg（both r=0.999 7）;RSDs of precision, stability and reproducibility tests were all lower than 1%. Average recoveries were 99.39%（RSD=1.62%,n=6） and 99.51%（RSD=1.05%,n=6）. CONCLUSIONS：With A. lancea increased, the content of alkaloid decreased when P. chinense was compatible with A. lancea.