高压CO_2对枯草芽孢杆菌杀菌模型及其优化

Optimum process of Bacillus subtilis of high pressure inactivation in food by response surface methodology

以枯草芽孢杆菌为主要研究对象,杀灭对数值为衡量高压CO2杀菌效果的指标。在单因素实验基础上,选取压力、温度和保压时间为自变量,杀灭对数值为响应值。通过响应面方法中的Box-Behnken模式,研究各自变量及其交互作用对杀灭对数值的影响,模拟得到二次多项式回归方程的预测模型。确定最佳条件为压力30MPa,温度48℃,时间90min,实际测得的枯草芽孢杆菌杀灭对数值为5.22,与模型预测值5.44相比,实验平均相对误差为2.32%;采用响应曲面优化法建立的回归模型准确可行,优化得到的工艺参数可以应用。

Bacillus subtilis was as the research object,with the killing log value as a measure indicator. Three inactivation parameters including pressure,temperature and pressure holding time were optimized using response surface methodology （RSM）. Box-Behnken design was applied to evaluate the effects of three indepent variables on the inactivation of Bacillus subtilis spores. The simulated quadratic polynomial regression equation of prediction model was set up. The optimum process parameters for inactivation of Bacillus subtilis were obtained as follows.pressure 30Mpa,temperature 48℃,pressure holding time 90min. Under the condition of killing log value was 5.22,compared to the theoretical value,the relative error of 2.32%. The adequacy of the model equation for predicting the optimum response values was verified effectivelu by the validation data.