氨基酸检测方法的进展和现状

Progress in determination methods of amino acid

氨基酸是构成蛋白质的基础结构,是生物体内不可缺少的营养成分,几乎与一切生命活动相关。氨基酸的准确测量,在氨基酸生产的质量控制、临床疾病诊断、饲料的品质评价等领域具有重要意义。许多国内外研究机构都在研究快速、准确的检测方法。常见的检测方法有离子交换色谱法、气相色谱法、高效液相色谱法、毛细管电泳、色谱质谱联用等。比较全面地总结了氨基酸的检测方法及其研究进展。

Amino acids are the building blocks of proteins and the necessary nutrition compositions in organisms. Almost of all life activities are related to amino acids. The accurate measurement of amino acids are very important for amino acid production control, clinical disease diagnosis and feed quality evaluation. Many studies are focused on fast and accurate methods. Conventional methods for determination of amino acids include ionexchange chromatography, gas chromatogra phy, highperformance liquid chromatography, capillary elec trophoresis, and chromatographymass spectrometry. In this work, the progress in determination methods of amino acids in recent years were reviewed.