芝麻核心种质株高构成相关性状的遗传变异及关联定位

Genetic variation and associated mapping for traits related to plant height constitutions in core collections of sesame (Sesamum indicum L.)

以我国216份芝麻核心种质为材料,分析芝麻株高及其构成相关性状的变异、相互关系等,利用79对EST-SSR、AFLP和SRAP引物对供试材料基因组进行检测并进行标记-性状关联分析,检测群体中与株高构成性状相关的DNA变异位点。结果表明,株高及其构成性状的变异均呈现连续变化,变异丰富,变异系数均在10%以上;相关性分析和多元回归拟合株高及其构成因素间的关系表明,主茎始蒴高度、主茎果轴长度是影响株高差异的主要因素。利用GLM(Q)和MLM(Q+K)模型共检测到34个变异位点同时与供试群体两年的株高构成显著关联,对表型变异解释率在1.89%～5.29%,平均为2.82%,其中与主茎始蒴高度显著关联的标记位点M20E12-3被2种模型均检测到。此外,研究发现株高构成因素受基因型、环境和两者互作影响,且均达到极显著水平。

216 accessions in sesame（Sesamum indicum L.） core-collection were used to study the variation and interrelationship of plant height constitutions.All accessions were scanned with 79 pairs of EST-SSR,AFLP and SRAP primers,and the loci related to plant height constitutions were predicted by association mapping.Result showed that all traits of plant height constitutions presented a trend of continuous change.They had abundant variation types and the CV（coefficient of variation）were all above 10%.Correlation analysis and multiple regression analysis showed that stem height from base to first capsule and capsule zone length were the main factors affecting plant height.In total,34 loci related to plant height constitutions in both 2010 and 2011 were detected by association analysis based on GLM（Q） model and MLM（Q） model.These loci explained 1.89% to 5.29% of phenotypic variation with average of 2.82%.Among these loci,two were pleiotropic that affected two traits respectively detected by GLM（Q） model,only one locus M20E12-3 associating with stem height from base to first capsule was identified by both models.Result also showed that sesame plant height constitutions were significantly affected by genotype,environment and their interaction.