丁酸对人肺癌细胞的生长、细胞周期和形态的影响

Effects of Butyric acid on the Growth, Cell Cycle, and Morphology of Human Lung Cancer Cells

运用鉴别细胞群体生物学特性的常规方法和流式细胞光度术(FCM),我们研究了正了酸对人肺癌细胞(PLA-801D)的某些生物学作用。当培养液中含2或3mmol/L丁酸时,细胞分裂和增生受到明显抑制,群体倍增时间几乎延长1倍,细胞周期行进受阻,大多数细胞阻断在G1期,平皿集落形成率从23.9％降为1.9％,饱和密度从237.4万/ml降至48.8万/ml,细胞群体提前3d进入饱和状态。与此同时,细胞变扁平,几乎丧失叠堆生长能力,表面膜活动减弱,核形趋于规则,染色质颗粒分散变稀疏,细胞间粘着连接明显增多。以上发现提示丁酸可使PLA-801D肺癌细胞获得一些相对正常的生长特性和表型,显示丁酸对该细胞有一定程度的分化诱导作用和潜在的抗癌作用。

The effects of butyric acid on cloned human lung cancer cells (PLA-801D) were observed with flow cytometry (FCM) and the conventional methods to identify the biological characteristics of cell lines. Butyric acid ( 2 or 3 mmol/L)treatment could result in an inhibition of the cell division and the growth rate of the cells,the blocking of the cell cycle progression in the Gl phase,a prolongation in doubling time, a shortening in saturation time, a decrease of saturation densities,and a reduction of plating efficiency. At the same time,there were some normal phenotypes of the cells such as flattening, tendency to grow in a monolayer,weakening of surface membrane activities, regularization of nuclei, and increasing of intercellular junctions.These findings suggest that after the treatment of butyric acid, the cancer cells (PLA-801D) appeared to acquire relatively normal growth properties and phenotypes. Thus, butyric acid, to a certain extent, can induce differentiation of PLA-801D cells, and be a potential anticancer agent.