无机三氧化聚合物对人牙乳头干细胞TNF-α和IL-1表达的影响

Effects of mineral trioxide aggregate on the expression of TNF-α and IL-1 in human stem cells from apical papilla

目的探讨无机三氧化聚合物（mineraltrioxideaggregate，MTA）对人牙乳头十细胞（stemcellsfroma·alpapillaeelkSCAPs）早期炎性蝌子TNF-a和IL-l表达的影响。方法酶消化法分离增养人SCAPs，经免疫磁珠筛选后，采川2mg／m1．MTA刺激1d、3d后，酶联免疫吸附法（enzymelinkedimmunosodentassay，ELlSA）检测其上清液中肿瘤坏死因子-a（tunlornecrosisfactor—a，TNF—01）、白细胞介素1（Interleukin—l，IL-1）的含量，采川实时定艟PCR（real—timeRT—PCR）检测各组细胞TNF-a、IL—la、1L-1B基因的表达水平。结果经MTA刺激ld和3d后，SCAh细胞上清液rfJTNF-a、浓度均较正常对照组低（P〈0．01），细胞对照组低（P〈0．01）．结论MTA可抑制SCAPs早期炎性因子TNF-a 、IL—1的表达，通过降低细胞的炎性因子来释放来减轻组织的炎性反应。

Abstract Objective To determine the effects of mineral trioxide aggregate （MTA） on the expression of TNF-a and IL-1 in human stem cells from apical papilla （SCAPs）. Methods SCAPs were isolated by magnetic activated cell sorting （MACS） and cultured in oL-MEM medium containing 2 mg/mL MTA for 1 day and 3 days. Enzyme linked immunosorbent assay （ELISA） was applied to examine the TNF-a and IL-1 in the culture supematant of SCAPs. Quantitative RT-PCR was used to determine the gene expression of TNF-a, IL-la, and IL-1β in SCAPs. Results The expression levels of TNF-a and IL-1 at both protein expression and mRNA transcription were inhibited （P 〈0.01） when the cells were treated with MTA. Conclusion MTA can inhibit the expression of early-stage inflammatory cytokines and relieve the inflammation via reducing the secretion of inflammatory cytokines.