血清IL-1β、IL-6、IL-18水平与2型糖尿病大鼠胰岛素抵抗的关系

Serum IL-1β,IL-6 and IL-18 levels are associated with insulin resistance in rats with type 2 diabetes mellitus

目的探讨白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、白细胞介索18(IL-18)与2型糖尿病大鼠胰岛素抵抗的关系。方法 30只SPF级Wistar大鼠随机分为对照组(15只)和糖尿病组(15只)。高脂饲料配合小剂量链脲佐菌素30mg/kg静脉注射建立2型糖尿病大鼠模型。4周后观测大鼠空腹血糖(FBG)、血清胰岛素(FINS)及血清IL-1β、IL-6、IL-18水平,并计算胰岛素抵抗指数(HOMA-IR)。结果 4周后糖尿病组大鼠FBG、FINS和HOMA-IR均高于对照组(均P<0.01);且血清IL-1β、IL-6、IL-18水平均高于对照组(均P<0.01)。结论血清IL-1β、IL-6、IL-18水平升高与2型糖尿病胰岛素抵抗的发生有关;IL-1β、IL-6、IL-18的同时升高可间接反映2型糖尿病及胰岛素抵抗的病情变化。

Objective To investigate the association of serum interteukin-1β （IL-115）, interleukin-6 （IL-6） and inter- leukin-18 （IL-18） levels with insulin resistance in rats with type 2 diabetes mellitus. Methods Thirty SPF Wistar rats were divided randomly into diabetic group（n=15） and control group（n=15）. The diabetes mellitus was induced by feeding with high-fat diet and injection of streptozotocin （30mg/kg）. The fasting blood glucose （FBG）, fasting insulin （FINS） and serum levels of IL-1β, IL-6, IL-18 were measured 4 weeks after model establishment, and homeostasis model assessment of insulin resistance（HOMA-IR） was calculated. Results Compared to control group, the serum levels of FBG, FINS and HOMA-IR were significantly increased in diabetic group（P〈0.01）. The serum levels of IL-1β, IL-6, IL-18 of diabetic group were remarkably increased as compared to those in control group（P〈0.01）. Conclusion The serum IL-1 β, IL-6, IL-18 levels are associated with insulin resistance in type 2 diabetes mellitus.