异种脱钙骨基质加释放系统诱导成骨的~3H-TdR、^(45)Ca示踪研究

A Study on the Osteogenesis Induced by HeterogenousDecalcified Bone Matrix and Delivery System with^3H-TdR and ^(45)Ca Tracing in Rats

采用Wistar大白鼠105只,于双侧股四头肌内随机植入异种脱钙骨基质(简称HDBM)。HDBM+CaCl_2+Na_2HPO_4+KH_2PO_4、HDBM+CaSO_4+Na_2HPO_4+KH_2PO_4、HDBM+NaCl。通过示踪剂~3H-TdR、^(45)Ca连续检测,结果证明CaSO_4+Na_2HPO_4+KH_2PO_4或CaCl_2+Na_2HPO_4+KH_2PO_4均是HDBM诱导成骨的良好促进剂,用其处理的HDBM具有很好的诱导成骨作用,但前者比后者更优。

one hundred and five Wistar rats were randomized into 3 groups and heterogenous decalcified bone matrix (HDBM) + CaCl_2+Na_2HPO_4+KH_2PO_4, HDBM + CaSO_4+Na_2HPO_4+KH_2PO_4, and HDBM + NaCl were implanted into the quadriceps muscle of both thighs of the animals respectively. They were monitored with ~3H-TdR and ^(45)Ca tracing to evaluate the efficacy of the 3 delivery systems to enhance osteogenesis. It was found that coadministration of CaSo_4, Na_2HPO_4, and KH_2PO_4 could significantly enhance the osteogenesis induced by HDBM. It is concluded that HDBM and the coadministration of Na_2HPO_4, KH_2-PO_4, and CaS0-4/CaCl_2 together will be much more effective to induce osteogenesis than HDBM is used singly.