芦笋提取物抗疲劳及耐缺氧活性研究

Study on anti-fatigue function and hypoxia function of asparagus extracts

目的:研究芦笋提取物抗疲劳及耐缺氧活性。方法:以75%乙醇为溶媒,超声提取芦笋,获得芦笋乙醇提取物,对提取物中总皂苷、总多糖、氨基酸进行含量分析;整体口服给药实验观察芦笋乙醇提取物对小鼠抗疲劳及耐缺氧功能的影响,给药剂量分别为25、100、400mg/kgmb。结果:给药14d后,小鼠负重游泳时间均显著延长(低、高剂量组p<0.01,中剂量组p<0.05);给药30d后,无负重游泳30min小鼠血乳酸含量显著下降(低、中剂量组p<0.01,高剂量组p<0.05),血尿素氮(BUN)含量没有显著性变化(p>0.05),肝脏谷胱甘肽过氧化物酶(GSH-PX)含量显著升高(低、中剂量组p<0.05,高剂量组p<0.01),过氧化物歧化酶(SOD)含量显著升高(低剂量组p<0.01,中、高剂量组p<0.05),高剂量组丙二醛(MDA)含量显著下降(p<0.05);给药30d后,高剂量组小鼠常压密闭存活时间显著延长(p<0.05),注射盐酸异丙肾上腺素后,各剂量组小鼠耗氧量及存活时间均无显著变化。结论:芦笋乙醇提取物有明显的抗疲劳功能及一定的耐缺氧功能。

Objective= Study the anti- fatigue function and hypoxia function of asparagus extracts. Methods= asparagus scrap was ultrasonic extracted with 75% ethanol.The contents of total saponin, polysaccharide and amino acids in the extract were determined.The effect of the ethanol extract from stem bottom of Asparagus on Anti -fatigue function and Hypoxia function of normal mice were studied by oral administration at three dose levels（25, 100,400mg/kg m b）.Results：After 14d of taking drugs,weight loading swimming time of mice significantly extend （low, high close group p 〈 0.0],the middle dosage group p 〈 0.05）.After swimming 30min without weight loading in taken drugs for 3ed, the accumulation of blood lactic acid in mice significantly decreased （ low, middle dosage group p 〈 0.01, high dose group p 〈 0.05）, while the blood urea nitrogen （BUN） content showed no significant change （ p 〉 0.05 ）, Glutathione peroxidase （ GSH- PX） content significantly increased （low, middle dose group p 〈0.05, high dosage group p 〈 0.01 ）, Superoxide dismutase （SOD） content significantly increased （ low, close group p 〈 0.01, middle, high dosage group p 〈 0.05 ）, the Malondialdehyde （MDA） content of high dose group significantly decreased（p 〈 O.05）.After taking the medicine for 30cl continuously, the hypoxia- resistance time of high doses of mice significantly extended （ p 〈 0.05 ）, after injection hydrochloric acid isoproterenol, each close group of mice oxygen consumption and survival time had no significant change.Conclusion：the ethanol extract from stem bottom of asparagus had obviously anti-fatigue function and certain hypoxia function.