硫化氢中毒对大鼠肺亚细胞结构标记酶的影响

Effects of Hydrogen Sulfide Intoxication on Marker Enzymes of Pulmonary Subcellular Organelles in Rats

大鼠吸入H_2S 200ppm3h中毒,测定了中毒后3,12,24,48.72和96h血清及防线粒体-溶酶体、细胞核、细胞浆及微粒体中LDH、ACP、CPK及Na^+-K^+-ATP酶活性的变化。结果表明,H_2S吸入中毒后亚细胞水平的肺损伤具有发生快、维持时间长等特点,中毒后3h即有溶酶体线粒体破裂、细胞能量代谢抑制、细胞破裂及标记酶入血,12h亚细胞损伤达高峰,随后渐恢复,72h恢复至正常水平,血清酶活性变化与亚细胞水平的变化高度相关,提示血清酶的变化源于肺损伤,因此,测定血清酶的变化能较好地反映亚细胞水平的肺损伤。

The changes of the marker enzymes of pulmonary subcellular organelles were determined in the 3rd, 12th, 24th, 48th, 72nd, and 96th hour after the rats were exposed to the air containing 200 ppm of hydrogen sulfide(H2S) for 3 hours. It was found that H2S injury on the subcellular organelles was characterized by the early occurrence of the rupture of lysosomes and mitochondria, the suppression on the energy-related enzymes, and the appearance of pulmonary subcellular marker enzymes in the serum. The pulmonary subcellular injury began to appear in the 3rd hour and the most obvious injury occurred in the 12th hour after exposure. Thereafter, the subcellular injury recovered gradually. In the 72nd hour after exposure, the activities of all the marker enzymes returned to the control level. The changes of serum marker enzymes were highly correlated with those in pulmonary subcellular organelles. The findings suggest that the occurrence of marker enzymes in the serum after H2S intoxication originates from pulmonary injury.Therefore determination of the activities of serum marker enzymes can be used as an indicator of pulmonary subcellular injury H2S intoxication produces.