γ射线辐照对SD大鼠淋巴细胞基因表达谱的影响

Effects of γ-rays irradiation on gene expression profiles of SD rat lymphocyte

研究了2.0 Gyγ射线照射对大鼠血液淋巴细胞基因表达谱变化的影响。通过体内、外实验,用2.0 Gy60Coγ射线照射后24 h,提取血液中淋巴细胞的RNA,用基因芯片技术检测淋巴细胞基因表达谱的变化。结果表明,照射后24 h,体外和体内共同的差异表达基因有1322条,其中下调表达有497条,上调表达有633条,有192条表达不一致。结合基因功能注释(Gene ontology,GO)数据库,共同涉及到的生物过程、细胞组分、分子功能分别有53、19、28个。结合KEGG数据库,共同包含的pathway有12条通路。此外,还发现了Serpinb2、Creb3l2、Pacrg、Vamp1和Il16等与辐射相关的基因。

The gene expression changes of rat blood lymphocytes after 2.0 Gy γ-ray irradiation were studied. At 24 h after in vitro and in vivo irradiation with 2.0 Gy 60Coγ-ray, RNA in blood lymphocyte was extracted and the changes in the gene expression profiles of lymphocytes were detected. The results showed that at 24 h after irradiation the common genes being significantly expressed in in vitro and in vivo comprised 1,322 genes, including 497 down-regulated genes and 633 up-regulated genes. Further, 192 genes are inconsistent. Referring to the GO database, there are 53, 19, 28 functional clusters involved in biological processes, cellular components and molecular functions, respectively, and there are 12 pathways involved. In addition, some radiation-related genes such as Serpinb2, Creb312, Pacrg, Vampl, I116, and others were also found.