HPLC同时测定枸骨叶中熊果酸和齐墩果酸的含量

Determination of Ursolic Acid and Oleanolic Acid in Ilicis Cornutae Folium by HPLC

目的:建立枸骨叶药材中熊果酸、齐墩果酸的含量测定方法。方法:采用高效液相色谱法,色谱柱:Extend-C18(4.6mm×250mm,5μm),流动相:甲醇-0.1moL L-1醋酸铵溶液(83 17),流速为1.0mL min-1,柱温30℃,检测波长210nm。结果:熊果酸在23.6-236.0μg mL-1线性关系良好(r=0.999 9),平均回收率为98.84%,RSD为0.75%。齐墩果酸在10.42-104.20μg mL-1线性关系良好(r=0.999 9),平均回收率为98.87%,RSD为1.00%。结论:该方法简单,准确,且专属性强,可作为枸骨叶药材质量控制方法。

Objective： HPLC method was established for determination of ursolic acid and oleanolic acid in Ilicis Cornutae Folium. Method： HPLC was carried out on Extend-C 18（4.6mm×250mm,5μm） at 30℃ using methanol-0.1 moL . L-1 ammonium acetate（83：17） as mobile phase with a flow rate of 1.0 mL . min^- and detection wavelength was at 210nm.Result： Linear correlation（r=0.9999） of the peak area and the concentration of ursolic acid over the concentration range 23.6-236.0μg . mL^-1 was obtained.Linear correlation（r=0.999 9）of the peak area and the concentration of oleanolic acid over the concentration range 10.42-104.20μg . mL^- was obtained. The average recovery was 98.84%,98.87% and RSD was 0.75%,1.00%.Conclusion： The method is simple,rapid and accurate. It can be used for the quality control of Ilicis Cornutae Folium.