荧光定量PCR法与常规法检测女性解脲脲原体、沙眼衣原体的比较

Comparison of fluorescent quantitative PCR method and conventional method for detection of ureaplasma urealyticum and chlamydia trachomatis

目的应用荧光定量PCR法(FQ-PCR)与常规法[(用培养法检测女性分泌物解脲脲原体(UU)、用金标法检测沙眼衣原体(CT)],探讨两种方法的阳性检出率有无显著性差异以及FQ-PCR法的特异性和敏感性。方法对临床上疑似非淋菌性尿道炎的84例患者,采集宫颈外分泌物,同时运用FQ-PCR法和常规法检测UU、CT,并对其结果进行统计分析。结果 84例患者中,FQ-PCR法和培养法检测UU的阳性率分别为53.6%(45/84)和63.1%(53/84);FQ-PCR法和金标法检测CT的阳性率分别为10.7%(9/84)和2.4%(2/84)。经统计学分析,两种方法对CT的阳性检出率差异有显著性(Χ2=5.14,P<0.05),而UU差异无显著性(Χ2=3.5,P>0.05)。FQ-PCR与培养法检测UU相比,特异性达90.3%,敏感度达79.2%;而与金标法检测CT相比,特异性高达91.5%,敏感度高达100.0%。结论 FQ-PCR法与培养法在UU检测中阳性率相当,而在CT的检测中FQ-PCR法阳性率明显高于金标法,且具有较高的特异性和敏感性。

Object To detect in non-gonoeoccal urethritis by methods of fluorescence quantitative PCR （FQ-PCR） and conventional method,and to discuss two methods of positive rate have no significant difference as well as FQ-PCR specificity and sensitivity. Methods For clinically suspected non-gonococcal urethritis in 84 patients, urethral or exter- nal cervical secretions collected,using both FQ-PCR and conventional method for the detection of ureaplasm urealytic- umthe and chlamydia trachomatis,and the results of the statistics,analysis. Results For 84 cases of patients,FQ- PCR and culture method showed positive rates of 53.6% （53/84） and 63.1% （53/84） in UU detection, FQ-PCR and gold-labeled method indicated positive rates of 10.7 % （9/84） and 2.4 % （2/84） in CT detection, respectively. By sta- tistical analysis, the positive rate of CT was obviously statistical different between FQ-PCR and conventional method （ XZ=5.14, P d0.05）,but UU was not（ X 2：3.5, P ~0.05）. FQ-PCR and culture method for the detection of UU, the specificity was 90.3%, the sensitivity was 79.2% ; and compared with the gold-labbled method for detection of CT ,specificity was as high as 91.5 %,sensitivity was up to 100.0 %. Conclusion FQ-PCR was equivalent to cul- ture method detected in positive rate of , but FQ-PCR was significantly higher than the gold-labbled method in the de- tection of CT, and has a high specificity and sensitivity.