摘要
目的建立毛支清口服液中tortoside A的定量测定方法。方法采用高效液相色谱(HPLC)法,选用Venusil MPC18分析柱(250mm×4.6mm,5μm),乙腈-水(18∶82)为流动相,检测波长为207nm,体积流量为1.0mL/min,柱温30℃。结果tortoside A进样质量浓度为1.76～35.20μg/mL与峰面积呈良好的线性关系,回归方程Y=83335X-47442,r=0.9997,平均回收率为101.53%,RSD值为2.50%。结论所建立的HPLC法简便、准确、灵敏度高、重复性好,可控制毛支清口服液的质量。
Objective To establish a method for the determination of tortoside A in Maozhiqing Oral Liquid （MOL）. Methods The tortoside A was determined by high performance liquid chromatography （HPLC）. The Venusil MP C18 column （4.6 mm × 250 mm, 5 μm） was used, the mobile phase was acetonitrile-water （18：82）, the detection wavelength was 207 nm, the flow rate was 1.0 mL/min, and the column temperature was 30 ℃. Results Tortoside A has a good linear relationship with the peak area within the range of 1.76 —88.00 μg/mL with an regression equation Y = 83 335X-47 442 （r = 0.999 7）. The average recovery was 101.53%, and RSD was 2.50%. Conclusion The established method of HPLC could be used for the quality control of MOL for its simplicity, accuracy, high sensitivity, and good reproducibility.
出处
《药物评价研究》
CAS
2013年第3期203-205,共3页
Drug Evaluation Research
基金
国家自然科学基金资助项目(81073151)
