大鼠脊髓背根及其终末可塑变化的形态学研究

Morphometric study of the plasticity of nerve fibers of spared spinal dorsal root and its terminals in rats

本文利用电镜体视学技术研究了大鼠保留背根(L_4)内神经纤维与L_3脊髓节段内神经终末的数目。同时应用银染技术显示了脊髓内神经纤维的分布和密度。光镜显示银染的神经纤维在脊髓两侧的分布和密度无区别。电镜计量表明,保留背根内无髓神经纤维的数目明显增加,其直径也有增粗的趋势。Ⅲ板层内简单终末和复合终末的数目均可在术后恢复至正常水平,同时也增粗变大。本文对这些变化做了初步讨论。

The number of nerve fibers in the spared dorsal root of the L4 spinal nerve and the number of nerve terminals in the Lamina Ⅲ of the segment of the spinal cord between L2 and S1 were determined with electron microscopic morphometry,and the distribution and density of nerve fibers in the spinal cord were observed with silver staining.It was found that the distribution and density of nerve fibers in the spinal cord showed no difference between the operated and the control sides under optical microscopy;morphometric analysis revealed a significant increase of the number of unmyelinated fibers and a tendency to increase of their diameter;and the number of both simple and complex terminals in the Lamina Ⅲ of the spinal cord could return to the normal level on the 35th day after operation with a significant increase of their diameter.The importance of these changes was preliminarily discussed.