心肌梗死后大鼠periostin蛋白表达量与瑞舒伐他汀干预作用研究

Effects of rosuvastatin on expression of periostin protein in rats with acute myocardial infarction

目的观察心肌梗死后大鼠periostin(Pn)表达与瑞舒伐他汀的作用关系,探讨瑞舒伐他汀在梗死后心肌重构中的作用机制。方法雌性54只远交群(SD)大鼠,随机分为正常对照组、模型组、瑞舒伐他汀组,每组18只。其中36只(SD)大鼠,结扎左前降支建立急性心肌梗死模型,随机分为急性心肌梗死(AMI)对照组和瑞舒伐他汀(ROS)干预组;另18只为假手术组(Sham),只穿线不结扎。ROS组大鼠以6 mgROS/(kg·d)灌胃,另两组以等量清水灌胃。4周后测定各组大鼠胶原容积分数(CVF)、periostin蛋白及TGF-β1和periostin-mRNA表达量。结果与AMⅠ组相比,ROS组心肌CVF、periostin蛋白及TGF-β1和periostin-mRNA表达量均显著降低(P<0.05),ROS组和AMⅠ组相关指标均显著高于Sham组(P<0.05)。结论瑞舒伐他汀能够显著降低受损心肌中periostin表达水平,可能是其改善心肌重塑的机制之一。

Objective To evaluate the effects of rosuvastatin on expression of periostin （Pn）protein in rats with acute myocardial infarction and to investigate the improvement of myocardial reconstruction mechanism. Methods Female adult SD rats were randomly divided into sham group, model group and benazepril group （18in each group）. Thirty-six female SD rats with ligated LAD were used to establish models of acute myocardial infarction （AMI）, twenty four hours later, the models were randomly divided into acute myocardial infarction （AMI） as control group and rosuvastatin intervention （ROS） group.Another 18 rats were tested with pierced but not ligated LAD as sham-operated group （Sham）. Rats in ROS group were given rosuvastatin by gavage at 6 mg/（kg·d） dose levels to soluble in 1 ml clear water, whereas rats in the other two groups were given 1 ml clear water each day. Four weeks later, compare the differences of collagen volume fraction （CVF）, periostin protein, TGF-β1 mRNA and periostin mRNA. Results Myocardial CVF, periostin protein, TGF-β1 mRNA and periostin mRNA were reduced in the ROS group compared with rats in the AMI group （P0.05）, both ROS group and AMI group were significantly higher than those in the Sham group （P0.05）. Conclusion Rosuvastatin can reduce the expression of periostin in the damaged myocardial, may be one of the mechanism of myocardial remodeling improvement.