人胎肝再生刺激物质对大鼠中毒性肝坏死的治疗作用

Effects of human fetal hepatic stimulator substance on D-galac-tosamine-induced liver necrosis in rats

本文报道了人胎肝再生刺激物质(h-HSS)、人胎肝胞溶质(h-FLC)对D-氨基半乳精中毒大鼠的存活率和肝功能的影响,和对h-HSS的理化性质及其分子量进行了初步分析与测定。结果发现:h-HSS和h-FLC均能显著提高中毒大鼠的存活率;显著降低其血清OCT和mAST活性,提高HPT-A及促进~3H-TdR掺入肝内DNA的速率。加热95℃、30min,h-HSS仍保留其活性,而经胰蛋白酶处理后,其活性丧失。在SDS-PAGE上,h-HSS显示1条主带和3条次带,各带分子量依次为1.5、2.4、3.4和4.0万道尔顿,主带相对含量占47.57％。

The effects of human fetal hepatic stimulator substance (h-HSS) and liver cytosol (h-FLC) on the survival rate,the intensity of liver damage,and liver regeneration in rats with liver necrosis induced with D-galactosamine were observed.The physico-chemical properties of h-HSS were preliminarily determined.It was found that both h-HSS and H-FLC could markedly increase the.survival rate of the rats with toxic liver necrosis,significantly decrease the serum level of ornithine carbamoyl transferase and mitochondria aspartic trans-aminase,improve the hepaplastin activity,and elevate the incorporation rate of 3H-thymidine with liver DNA.h-HSS was stable after heated to 95℃ for 30 minutes,and sensitive to trypsin treatment.It demonstrated one major band at 15 000 daltons and 3 minor ones at 24 000,34 000,and 40 000 daltons respectively on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.The relative content of the major band was 47.57%.