黄芩愈伤组织中黄芩苷与过氧化物酶活性相关性分析

Analysis of the Correlation Between Baicalin and Peroxidase Activity in the Callus of Scutellaria baicalensi Georgi

以黄芩愈伤组织为试材,研究了其生长过程中过氧化物酶活性变化规律及其与黄芩苷次生合成之间的关系。结果表明:在黄芩愈伤组织生长周期(27d)内,过氧化物酶的活性高低与黄芩苷的积累呈正相关性。在黄芩愈伤组织生长周期的前15d,愈伤组织处于生长期,过氧化物酶活性低水平表达,黄芩苷合成缓慢;15～21d,愈伤组织处于稳定期,过氧化物酶的活性高水平表达,并到达最大值,期间黄芩苷迅速合成,也达到最大值;21～27d,愈伤组织进入衰老期,而此时的过氧化酶活性降低,黄芩苷合成几乎停止。采用L9(34)正交表优化培养基中6-BA、碳源、氮源、pH的配比,筛选过氧化物酶活性表达最优的培养条件为:6-BA 2mg/L,碳源:20g蔗糖+10g葡萄糖,氮源(NH4+)30mmol/L,pH 6.0。

Taking the callus of Scutellaria baicalensis Georgi as material,the relationship between the variation of peroxidase activity and baicalin secondary synthesis in the growth process of the callus of Scutellaria baicalensis Georgi were studied.The results showed that during the growth cycle（27 days） of the callus of Scutellaria baicalensis Georgi,the activity of the level of peroxidase and the accumulation of baicalin was positively correlated.During the former 15 days of the growth cycle of the callus of Scutellaria baicalensis Georgi,the skullcap callus was in its growth phase.The activity of the peroxidase express at a low level.The synthesis of baicalin was slow.During 15 to 21 days,the callus was in its stable phase.The activity of peroxidase express at high levels and reached its maximum,at the same time baicalin rapidly synthetised and reached its maximum.During 21 to 27 days,the callus was in its aging period,while the activity of peroxidase reduced.The synthesis of baicalin almost stopped.L9（3^4） orthogonal table was used to optimize the ratio of medium 6-BA,carbon sources,nitrogen sources,the pH.The optimal culture conditions were 6-BA 2 mg/L,carbon source：20 g sucrose＋10 g glucose,nitrogen source（NH4^＋） 30 mmol/L,pH 6.0.