无患子皂甙对瘤胃微生态的影响

Effect of Sapindoside on Rumen Microbiota

本研究探讨了添加无患子皂甙(SAD)对瘤胃微生态的影响。采用体外培养法,比较茶皂素(TS)、无患子提取物(SADE)和纯度为75%的无患子皂甙粉(SADC)对24 h培养液中微生物种群数量的影响。荧光定量PCR测定表明,TS和SAD均极显著降低了产琥珀酸丝状杆菌的数量(P<0.01),但显著提高了溶纤维丁酸弧菌的数量(P<0.05);甲烷菌的数量各处理间差异不显著(P>0.05),但TS和SAD显著降低原虫数量(P<0.05),且SAD组原虫数量极显著低于TS组(P<0.01)。DGGE图谱分析表明,SAD和TS基本不影响细菌区系,但增加了原虫区系多样性指数(P<0.05),且SAD降低了甲烷菌区系多样性指数(P<0.05)。结果表明:SAD与TS对微生物的作用机制相似,但SAD的作用效果优于TS,同时SAD可使瘤胃内的氢化程度提高。

The in vitro gas test was conducted to investigate the effect of Sapindoside of extract （SADE）, Sapindoside of company （SADC） and tea saponin （TS） on rumen microbiota. After 24h＇s incubation, the results of real-time quantitative PCR showed that relative to total bacterial 16S rDNA, the populations of Fibrobacter succinogenes was decreased （P〈0.01） and B. fibrisolvens was increased （P〈0.05） by TS and SAD treatments. The population of methanogens wasn＇t changed significantly （P〉0.05）, but protozoa population was decreased significantly （P〈0.05） in TS and SAD groups. Furthermore, protozoa was significant less in SAD group than that in TS group （P〈0.01）. DGGE profiles showed that bacteria community wasn＇t affected significantly by SAD and TS treatments, but the diversity index of protozoa community was increased （P〈0.05）. The methanogens community was decreased in SAD group （P〈0.05）. It is suggested that SAD and TS have similar effects on rumen microbiota, but the effects are more effective in SAD than that in TS, and tureen hydrogenation may be improved by SAD.