摘要
为了探测长期冷藏对蓝舌病样品的Real-time PCR检测结果和蓝舌病病毒活性的影响,本试验对18份4℃保存了10~18年的样品进行Real-time PCR检测,并对其中10份全血样品进行蓝舌病病毒分离和免疫酶染色,结果显示所有检测样品都为Real-time PCR阳性,从10份全血样品分离出5株蓝舌病病毒,通过免疫酶染色进一步证实为蓝舌病活病毒。研究结果提示冷藏蓝舌病病毒的活性高达18年,其稳定性超过目前现有的认识。
In order to understand the stability of blue-tongue virus (BTV)storage in cold room for long time, 18 tissue sam- ples which were kept in 4 ℃ for 10 to 15 years were test by Real-time PCR, and 10 blood samples which were kept in 4 ℃ for 15 years were subjected to virus isolation process, then was proved by monoclonal antibody staining,research results showed all old samples were positive in Real-time PCR, further more, 5 live virus were isolated from 10 old blood samples. The results suggested the stability of BTV was higher than we thought before.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第6期41-44,共4页
China Animal Husbandry & Veterinary Medicine
基金
"十二五"农村领域国家科技计划:<动物疫病分子诊断技术研究和产品创新>(2012AA101301-2)
国家公益性行业科研专项:<边境地区动物疫病防控技术体系研究>(200803026)
云南省应用基础研究计划项目:蓝舌病Ⅰ型病毒诱导绵羊产生IL-2和IFN的机制研究
关键词
蓝舌病
长期冷藏
稳定性
blue-tongue virus
storage in cold room
stability