绵羊痘病毒E3L蛋白N端结构和功能预测

Function Prediction and Structure Analysis of N-terminal of E3L Protein in Sheeppoxvirus

E3L蛋白是痘病毒的一类早期表达的非结构蛋白,其主要抑制宿主的先天性免疫,与病毒的致病力和宿主嗜性有关,但是目前对绵羊痘病毒E3L蛋白的研究较少。作者运用生物信息学的方法,用已知的E3L蛋白N端结构作为模板,并使用WebLab、SWISS-MODEL和ExPASY等在线软件预测其功能结构域和二、三级结构及氨基酸组成,对其作为Zα潜在家族成员的真实性、可靠性作出理论上的判断。分析结果表明,绵羊痘病毒E3L蛋白N端结构符合Zα家族蛋白的一些基本的结构特征,具有其他E3L蛋白N端相同的功能结构,为该蛋白质N端结构属于Zα蛋白家族提供了一些理论依据。

E3L protein is a kind of nonstructural protein of poxvirus expressed in early time. It can inhibit immunity of host cells and be ralated with virus virulence and tropism. However, E3L protein in sheeppoxvirus （SPPV） is rarely understood. We have predicted N-terminal of E3L protein in SPPV by bioinformatics methods as well as using the N-terminal of E3L pro- teins which have been predicted. And then the structural domains, secondary structures, tertiary structures and amino acid compositions of the above proteins have also been predicted using these online programs, such as WebLab, SWISS-MODEL and ExPASY tools. The bioinformatical analysis results show that the protein and other Za famliy proteins have the same basic structural characteristics and structural domains, implying that the N-terminal of E3L protein in SPPV might belong to Za famliy.