弯曲杆菌中具有高抑菌活性乳酸菌的筛选

Screening of Lactobacillus with Highly Antagonistic Activity against Campylobacter

使用微生态制剂来替代抗生素用于动物疫病防制是未来的发展趋势,而乳酸菌是目前微生态制剂最主要的成分。试验旨在筛选弯曲杆菌中具有高抑菌活性并耐受动物胃肠道环境的乳酸菌。从广东不同地区采集家禽粪便样品,对分离的乳酸菌进行生化鉴定、PCR鉴定及16SrDNA测序。同时,对分离菌株进行鸡源空肠弯曲杆菌、鸭源空肠弯曲杆菌和鹅源结肠弯曲杆菌的抑菌试验,以及模拟胃肠道环境的耐酸、耐胆盐和耐胰蛋白酶试验。结果表明,唾液乳杆菌R3对不同禽源的弯曲杆菌都具有高抑菌活性和耐受能力。因此,唾液乳杆菌R3为开发防控家禽弯曲杆菌疾病的微生态制剂提供了候选株。

Using probiotics replaced antibiotic for the treatment and prevention of animal in the future, and lactic acid bacte- ria （LAB） were the application of probioties in the main component. Test was aimed to screen LAB which had high bacterio- static activity to Campylobacter and tolerance animal gastrointestinal environment. Poultry excrement were acquired from Guangdong different areas, the isolated LAB were dealed with biochemical identification, PCR identification and 16S rDNA se- quencing. Meanwhile, we tried to study the antibacterial ability of them against Ca mpylobacter jejuni isolated from chicken, duck and Carnpylobacter coli from goose, then we further examed the bearing capability of the LAB under acid, bile salts and trypsin. The results showed that Lactobacillus saliva R3 had high＇bacteriostatic activity and tolerance ability to different birds source Campylobacter. So, Lactobacillus saliva R3 would be one of candidate strains that used as microecologics to treat poub try Carnpylobacter disease.