牛源破伤风梭菌的分离与鉴定

Isolation and Identification of Clostridium tetanus from Cattle

1头进行了瘤胃瘘管手术的黄牛发病死亡,临床症状表现为肌肉强直性痉挛。本试验取其手术伤口部位的炎性肌肉进行了细菌分离,分离到1株破伤风梭菌CT-JX-1。该分离菌株为具有顶端芽孢的革兰氏阳性杆菌;在普通琼脂培养基形成出灰白色、扁平、粗糙的菌落,菌落中心部分致密,外缘呈蜘蛛网状;该菌株能液化明胶,对多种糖均不发酵。

A cattle fallen ill and died after an operation on the rumen fistula, the clinical symptoms mainly presemted tetanic spasm. In this experiment, Clostridium tetanus was isolated from the muscle of surgical wound. The isolated Clostridium teta- nus CT-JX-1 is gram-positive bacteria with bacillus on the top, forming the gray, flat and rough-type colonies on a general agar medium, it was dense on central part of the colony, and the outer edge like a spider web, this strain could liquefy the gelatin, but could not ferment a variety of sugars.