高灵敏度酶联免疫法快速检测动物源性产品中氯霉素残留的研究

Study on Rapid Determination of Chloramphenicol Residue in Animal Products by High Sensitive ELISA

本试验旨在应用酶联免疫吸附法(ELISA)检测动物组织中氯霉素的残留。根据试剂盒操作说明对样本进行前处理,样品中的氯霉素与氯霉素酶结合物竞争结合酶标板微孔中固相化的氯霉素特异性抗体,根据酶催化显色剂显色的深浅来判断样品中氯霉素含量。试验结果显示,该方法对样品的检测限在0.1μg/kg以下;以20～300ng/kg浓度的氯霉素添加到动物源性产品中,其回收率范围74.4%～105.4%;变异系数在15%以下。结果表明建立的ELISA试剂盒能满足检测动物源性产品中氯霉素残留的需要。

In this study, an ELISA method for determination of chloramphenicol （CAP） residue in animal products was used. According to the instruction of ELISA kit, CAP from pretreatment samples which was competed with CAP-HRP, and could be combined antibody coated. The content of CAP could be measured by color produced by substrate solution and HRP. The limit of the detection method in animal products was less than 0.1 μg/kg, and recoveries ranged from 74.4 % to 105.4 for fortified samples at levels of 20 to 300 ng/kg with coefficient of variation values less than 15%. The ELISA kit could meet the requirement of detecting CAP residue in animal products.