谷氨酰胺对急性肺损伤大鼠HGF及KGF的影响

The effect of Gln on HGF and KGF of acute lung injury rats(ALI) induced by sepsis

目的探讨谷氨酰胺对脓毒症大鼠急性肺损伤时的肝细胞生长因子(HGF)及角细胞生长因子(KGF)的作用。方法采用盲肠结扎穿刺法复制大鼠脓毒症急性肺损伤模型,40只健康SD大鼠,分为3组,即对照组、手术组[采用盲肠结扎穿刺法]、谷氨酰胺治疗组[采用盲肠结扎穿刺法后静脉注射谷氨酰胺0.75 g/kg]。实验中手术组死亡6只,治疗组死亡3只,在手术6 h后手术组随机处死6只,24 h后处死剩下5只,治疗组药物处理6 h后处死6只,24 h后处死剩下的8只,4 000 r/min离心10 min,收集血清,置于-20℃冰箱保存待用。Elisa法测定血清中HGF和KGF的量。并进行各组织的病理学光镜检查。研究谷氨酰胺对血清中HGF和KGF的影响。结果与手术组比较,谷氨酰胺治疗组血清HGF和KGF浓度明显升高(P<0.05)。结论静脉给予谷氨酰胺能促进脓毒症大鼠急性肺损伤时HGF和KGF的分泌,促进受损组织的修复。

[ Objective ] To investigate the effect of Gln on HGF and KGF on acute lung injury （ALI） in rats in- duced by sepsis. [Method] The acute lung injury model （ALI） in Rats was induced with sepsis by cecal ligation and puncture. Forty healthy adult SD rats were randomly divided into control group, operation group, and treatment group by Gln. The operation group adopted cecal ligation and puncture, the treatment group received an intravenous infusion of 0.75 g/kg Gin after cecal ligation and puncture. In operation group, 6 rats were randomly killed 6h after operation, last 5 rats were killed 24 h after operation. In treatment group, 6 rats were killed 6 h after medication, last 8 rats were killed 24 h after medication. Serum was collected after experiment and kept in refrigerator at-20~C. HGF, KGF in the serum were detected by ELISA. Pulmonary tissue was examined by microscope. Tile effect of Gin on HGFand KGF in the serum was investigated. [Result] Compared with the operation group, HGF, KGF in serum of treatment group significantly increased （P 〈0.05）. [ Conclusion ] Intravenous administration of Gln can promote the secretion of HGF and KGF and the repair damaged tissues.