三种植物甾醇调控SRE元件和3T3-L1细胞脂肪化活性的作用

Effects of Three Plant Sterols on Activity of Sterol Regulatory Element and Adipogenesis of 3T3L-1

[目的]为了研究植物甾醇对脂质代谢的调控作用。[方法]利用所构建的SRE荧光素酶活性测定系统,体外考察了豆甾醇、β-谷甾醇和菜油甾醇3种常见的植物甾醇对SRE元件的调控活性。[结果]植物甾醇能够增强SRE元件活性。植物甾醇也可以增加3T3-L1细胞的脂肪化作用。[结论]植物甾醇可能正调控脂质的合成。

[Objective] The research aimed to study the regulating effect of plant sterol on lipid metabolism.[Method]Luciferase reporter vector pGL3-basic that contained one or three sterol regulatory element（SRE） was transfected into HepG2,and luciferase activity of three plant sterols（stigmasterol,β-sitosterol and campesterol） was analyzed.[Result] The plant sterols had positive regulator of SRE.And plant sterols could increase adipogenesis of 3T3L-1.[Conclusion] Plant sterols could increase adipogenesis by increasing SRE activity.