不同养殖密度下革胡子鲶胃肠道的定量组织学研究

Quantitative Histology Study on the Stomach and Intestines of Clarias gariepinus at Different Culture Densities

[目的]为革胡子鲶科学的高密度养殖和可持续发展提供参考依据。[方法]研究4种养殖密度(35、65、95、125 kg/m3)下革胡子鲶胃和肠道纹状缘厚度、柱状细胞高、壁厚、肌肉层厚以及肠道杯状细胞大小,分析不同养殖密度对革胡子鲶消化道的组织结构变化的影响。[结果]养殖30 d时,养殖密度仅影响胃和肠道中段的纹状缘厚度。35 kg/m3密度组的胃纹状缘厚度显著低于65、95和125kg/m3密度组。肠中段的纹状缘高在35、65和95 kg/m3密度组间无显著差异,但显著低于125 kg/m3密度组。养殖60 d时,35 kg/m3密度组的肠中段纹状缘显著厚于65、95和125 kg/m3密度组,但粘液细胞则显著小于65、95和125 kg/m3密度组,而65、95和125 kg/m3密度组间纹状缘高度和粘液细胞大小差异不显著。35 kg/m3密度组与65 kg/m3密度组、95 kg/m3密度组与125 kg/m3密度组肠中段和肠后段的肠壁厚和肌肉层厚无显著差异,但35和65 kg/m3密度组显著高于95和125 kg/m3密度组。[结论]养殖密度仅对革胡子鲶胃、肠道组织结构的少量测量指标有影响,与革胡子鲶的高耐受性和适宜高密度养殖的特点相一致。

[Objective] The research aimed to provide reference basis for scientific high-density culture and sustainable development of Clarias gariepinus.[Method] The thickness of striated border,height of columnar cells,thickness of wall and muscle layer in stomach and intestine,as well as the size of mucous cell in the intestines of C.gariepinus at four densities of 35,65,95 and 125 kg / m3 were studied.And the effects of different culture density on the organization structure changes of digestive intestines of C.gariepinus were analyzed.[Result] After culture 30 days,the culture density only affected the thickness of striated border in stomach and middle segment of intestine.The striated border thickness of stomach in the density group of 35 kg / m3 was significantly lower than that in the other densities groups（65,95 and 125 kg / m3）,.The striated border height in middle segment of intestine in the densities groups of 35,65 and 95 kg/m3 had no significant difference and they were significantly lower than that in the density group of 125 kg / m3.After culture 60 days,The striated border in middle segment of intestine in the density group of 35 kg / m3 was significantly thicker than that in other densities groups（65,95 and 125 kg / m3）,but the size of mucous cell was significantly smaller than that in other densities groups（65,95 and 125 kg / m3）.But the striated border height and the size of mucous cell among the densities groups（65,95 and 125 kg / m3） had no significant difference.The thickness of intestine wall and muscle layer in middle segment and posterior segment of intestines had no significant difference between 35 kg / m3 density group and 65 density group,95 density group and 125 kg / m3 density group.But the thickness of intestine wall and muscle layer in middle segment and posterior segment of intestines in the density groups of 35 and 65 were significantly higher than that in the density groups of 95 and 125 kg / m3.[Conclusion]The culture density only affected a few of measurement indices of stomach and intestines of C.gariepinus,which was accordant with the high tolerance and high density culture characteristics of C.gariepinus.