镉、铝互作胁迫对大麦幼苗生理代谢的影响

Effect of Cadmium and Aluminum Combined Stress on Physiological Metabolisms in Barley Seedlings

采用营养液培养试验,研究了不同浓度镉(Cd)、铝(Al)单独及互作胁迫下大麦幼苗叶片叶绿素、游离脯氨酸和还原型谷胱甘肽(GSH)含量及根系活力等重要生理代谢指标的变化.结果表明,除1μmol/L Cd单独处理外,其它胁迫均严重影响大麦幼苗代谢,表现为叶绿素含量和根系活力下降,游离脯氨酸含量上升,虽然50μmol/L Al处理促进叶片GSH积累,但5μmol/L Cd和100μmol/L Al处理下叶片中GSH的积累量均显著下降;Cd、Al复合胁迫对大麦幼苗代谢的影响强于单一Cd、Al处理,其中100μmol/L Al+5μmol/L Cd处理对植株的毒害作用最强.

The hydroponic experiments were focused on the effect of single and combined stress of cadmium and aluminum on the content of chlorophyll, free proline and glutathione in barley leaves, as well as the root activity. The results show that except for 1.0μmol/L Cd single treatments, all the stresses seriously disordered the physi- ological metabolisms of barley plants, thus, leaf chlorophyll content, root activity significantly decreased while free proline content markedly increased. The stresses of both 5.0 μmol/L Cd and 100 μmol/L Al notably decreased GSH content though 50 μmol/L A1 stress increased. Binary metal stress caused more serious metabolisms disorder, especially for the combined stress of A1 100 ＋ Cd 5.