miR-27a通过调控Sprouty2促进胰腺癌细胞PANC-1的生长

MiR-27a regulates the growth of pancreatic cancer cells PANC-1 by targeting Sprouty2

目的探讨miR-27a在胰腺癌细胞生长过程中的作用及相关机制。方法应用RT-PCR检测胰腺癌组织中miR-27a的表达水平;应用CCK-8生长曲线和软琼脂克隆形成实验检测miR-27a对胰腺癌细胞PANC-1生长能力的影响;应用双荧光素酶报告基因实验和Westernblot筛选miR-27a的靶基因。结果 (1)相比较于癌旁正常胰腺组织,胰腺癌组织中miR-27a表达显著上调;(2)抑制胰腺癌细胞PANC-1内源性miR-27a能够显著下调癌细胞的生长活性;(3)miR-27a能够直接调控Sprouty2基因3'UTR中的MRE序列;(4)抑制PANC-1细胞内源性miR-27a能够显著上调Sprouty2蛋白35%。结论 miR-27通过调控胰腺癌细胞PANC-1中Sprouty2蛋白表达发挥癌基因功能。

Objective To explore the function of miR-27a in pancreatic adenocarcinoma and the possible targets. Methods The level of miR-27a in pancreatic adenocarcinoma tissuses was detected by RT-PCR. The growth vitality of PANC-1 cells after transcient transfection of miR-27a inhibitor was detected by CCK-8 and soft agar colony formation assay. The possible targets of miR-27a were choosen by dual luciferase reporter gene assay and Western blot. Results （1）The expression level of miR-27a in pancreatic adenocarcinoma tissuses was up-regulated dramatically compared with the paired normal pancreatic tissuses. （2）The growth ability of PANC-1 cells was suppressed significantly after transcient transfection of miR-27a inhibitor. （ 3 ） miR-27a can regulate the MRE of Spmuty2. （4）The protein level of Sprouty2 was up-regulated significantly by 35% after transcient transfection of miR-27a inhibitor in PANC-1 ceils. Conclusion miR-27a was up-regulated in pancreatic adenocarcinoma, and it may function as an oncogene in PANC-1 cells by targeting Sprouty2.