男性乙型肝炎病毒感染者体液与精子中的病毒DNA

HBV DNA in body secretions and spermatozoa among 87 males infected by hepatitis B virus

作者报告87例男性[本人和(或)其配偶为乙肝感染者]的唾液、尿液,精清与精于中HBV DNA的检测结果,表明精清与唾液的斑点杂交HBV DNA阳性率分别为13.8％与10.3％,1例精清HBV DNA阳性者,其血中除抗-HBs外均阴性。发现另1例其配偶为HBsAg携带者的男性精于斑点杂交HBV DNA阳性,而血清中除抗-HBs外HBV感染标志均阴性。精清阳性者的血清HBsAg滴度与HBV DNA阳性率均高于阴性者,但无统计学意义;而精清阳性者的抗-HBc IgM检出率显著高于阴性者(P<0.01)。本研究结果初步支持HBV可通过精子种系途径进行传播的假设。

Determination of HBV DNA in saliva, urine, seminal fluid and spermatozoa among 87 males who or whose wives were infected by HBV were reported in this paper. The results showed that the positive rate of HBV DNA by spot hybridization was 13.8% in seminal fluid and 10.3% in saliva With spot hybridization, HBV DNA was also found in the spermatozoa of an individual. His wife was HBsAg carrier and HBV markers in his serum were negative except anti-HBs. The tilers of HBsAg and positive rate of HBV DNA in sera were higher in those with HBV DNA in seminal fluid than in those without HBV DNA, but there were not significant differences among them. The positive rate of anti-HBc IgM was higher among those with HBV DNA in their seminal fluids than among those without one (P<0.01). The present findings support the hypothesis about transmission of HBV via the germ line.