表面引发接枝聚合法制备抗蚜威分子表面印迹材料及其分子识别与结合特性研究

PREPARATION OF MOLECULARLY IMPRINTED MATERIAL OF PIRIMICARB BASED ON SURFACE-INITIATED GRAFT-POLYMERIZATION AND ITS RECOGNITION CHARACTER

基于表面引发接枝聚合,设计与建立了一种新的分子表面印迹(MIP)方法.先使用偶联剂γ-氨丙基三甲氧基硅烷(AMPS)对微米级硅胶微粒进行表面改性,制得改性硅胶AMPS-SiO2;在酸性水溶液体系中,凭借强静电相互作用,阴离子单体对苯乙烯磺酸钠(PSSS)被结合在模板分子抗蚜威周围;改性硅胶AMPS-SiO2表面的氨基与溶液中的过硫酸盐构成氧化还原引发体系,在硅胶微粒表面产生自由基,引发结合在模板分子周围的SSS及交联剂甲基双丙烯酰胺(MBA)在硅胶微粒表面发生接枝交联聚合,从而实现了抗蚜威分子的表面印迹,制得了抗蚜威分子表面印迹材料MIP-PSSS/SiO2.以残杀威为对比物,采用静态与动态两种方法,考察研究了分子表面印迹材料MIP-PSSS/SiO2对抗蚜威的分子识别特性与结合性能.研究结果表明,印迹材料MIP-PSSS/SiO2对抗蚜威分子具有特异的识别选择性与优良的结合亲和性,相对于残杀威分子,MIP-PSSS/SiO2对抗蚜威分子的识别选择性系数为9.373,显示出高的分子识别选择性.

Based on a surface initiated graft-polymerization method, a novel surface-molecular imprinting technique is designed and founded. The coupling agent y-aminopropyhrimethoxysilane （AMPS） was bond onto the surface of silica gel particles, and amino groups were introduced onto the surfaces of silica gel particles, obtaining the modified particles AMPS-SiO2. In aqueous solution,the molecules of the anionic monomer sodium styrene sulfonate （SSS） were first combined around pirimicarb molecule （a pesticide） as the template molecule by right of strong electrostatic interaction. A redox initiating system was constructed by the amino groups on AMPS-SiO2 and ammonium persulphate in the solution, and free radicals were produced on the surfaces of silica gel particles. These free radicals initiated the monomer SSS and the crosslinker N, N1- methylene bisacrylamide （MBA） to be graft-copolymerized and crosslinked, and the surface-imprinting of pirimicarb molecule was realized. After removing the template molecules, pirimicarb molecule surface-imprinted material MIP-PSSS/SiO2 was obtained. Propoxur, which is another pesticide and its chemical structure is similar to that of pirimicarb to a certain degree, was used as a contrasting substance, and both static and dynamic methods were adopted to study the molecular recognition character and the binding property of MIP- PSSS/SiO2 for pirimicarb molecule. The experiment results show that MIP-PSSS/SiO2 have specific recognition selectivity and excellent binding affinity for pirimicarb molecules, and its selectivity coefficients for pirimicarb molecules with respect of propoxur molecules is 9. 373, displaying high molecular recognition selectivity and excellent combination ability for pirimicarb.