摘要
目的探讨扬州地区门诊腹泻型肠易激综合征（D．IBS）与5-羟色胺受体3的亚型HTR3A、HTR3E基因非翻译区多态性的关系。方法采用聚合酶链反应-限制性片段长度多态性分析（PCR．RFLP）对450例D．IBS患者与300例健康对照者HTR3A基因5’端非翻译区C．-42C〉T（rs1062613）和HTR3E基因3’非翻译区c．＊76G〉A（rs62625044）进行研究。结果男女性患者C．-42C〉T多态性位点基因型分布较正常对照的差异有统计学意义（P〈0．05），且T等位基因的频率与正常对照比较均有显著增高（P〈O．05）；女性患者c．＊76G〉A多态性位点G／A基因型和A等位基因的频率较正常对照均显著升高（P〈0．05），男性两组间的差异无统计学意义（P〉0．05）；与HTR3A基因的rs1062613位点CC基因型及HTR3E基因的rs62625044位点GG基因型相比，TT基因型及GA基因型与D．IBS密切相关，风险系数分别为0．29（95％CI：0．14。0．61）、0．62（95％CI：0．39～0．99），差异均有统计学意义。结论T等位基因和GA基因型可能分别是D-IBS患者和D-IBS女性患者的易感因素之一。
Objective To investigate the relationship between diarrhea-predominant irritable bowel syndrome （D-IBS） and HTR3A, HTR3E gene polymorphism in Yangzhou, Jiangsu province. Methods Polymerase chain reaction （PCR） amplification and restriction fragment length pdymorpbism （RFLP） technique were used to detect the 5＇ -UTR variant c.-42C〉T of HTR3A and 3＇ -UTR variant c. ＊ 76G〉A of HTR3E on 300 healthy subjects and 450 patients with D-IBS. Results There was significant difference noticed between the D-IBS patients and the controls in the genotype of c.-42C〉T sites （P〈0.05）, while the frequency of T allele was significantly higher in both female and male patients than that in the controls （P〈0.05）. The frequencies of G/A genotype and A allele of c.＊ 76G〉A sites were significantly higher in the female-patient group than that in the controls （P^0.05） , however, there was no significant difference between those male patients and controls （P〉0.05）. When comparing to the CC genetype of rs1062613 site and GG genetype of rs62625044 site, the TT and GA genotype were closely related to the D-IBS, with the risk coefficients as 0.29 （95% CI： 0.14-0.61 ） and 0.62 （95%CI： 0.39-0.99）, with statistically significant differences. Conclusion T allele and GA genetype might respectively serve as the predisposing factors of D-IBS and on the female D-IBS patients.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2013年第7期721-724,共4页
Chinese Journal of Epidemiology
基金
江苏省研究生创新工程项目（CXZZ12_0918）
国家自然科学基金（81173392）
